RNA Sequencing Methods

Technological advances in the sequencing field support in-depth characterization of the transcriptome. There are now many  genome-wide RNA sequencing methods used to investigate specific aspects of gene expression and its regulation, from transcription to RNA processing and translation:

  • tag-based methods for studying transcription
  • alternative initiation and polyadenylation events
  • shotgun methods for detection of alternative splicing
  • full-length RNA sequencing for the determination of complete transcript structures
  • targeted methods for studying the process of transcription and translation

With the ensemble of technologies available, it is now possible to obtain a comprehensive view on transcriptome complexity and the regulation of transcript diversity.


de Klerk E, den Dunnen JT, ‘t Hoen PA. (2014) RNA sequencing: from tag-based profiling to resolving complete transcript structure. Cell Mol Life Sci [Epub ahead of print]. [article]