RNA Sequencing Sources Sought – Notice RFP HHS-NIH-NIDA-NIA-AM2975853


RNA Sequencing

Solicitation Number: RFPHHSNIHNIDANIAAM2975853


Agency: Department of Health and Human Services
Office: National Institutes of Health
Location: National Institute on Drug Abuse


Sources Sought
Added: Jun 03, 2013 12:50 pm

RNA Sequencing
Sources Sought Notice
Contracting Office Address
Department of Health and Human Services, National Institutes of Health, National Institute on Drug Abuse, Station Support Simplified Acquisitions, 9000 Rockville Pike, Bldg. 31, Rm. 1B59 Bethesda, MD, 20892, UNITED STATES

This is a Small Business Sources Sought notice. This is NOT a solicitation for proposals, proposal abstracts, or quotations. The purpose of this notice is to obtain information regarding: (1) the availability and capability of qualified small business sources; (2) whether they are small businesses; HUBZone small businesses; service-disabled, veteran-owned small businesses; 8(a) small businesses; veteran-owned small businesses; woman-owned small businesses; or small disadvantaged businesses; and (3) their size classification relative to the North American Industry Classification System (NAICS) code for the proposed acquisition. Your responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible.

Background Information
The Laboratory of Molecular Medicine and Neuroscience (LMMN) focuses on the disease Progressive Multifocal Leukoencephalopathy, which is caused by JC Virus. JC Virus causes lytic infection of cells of the central nervous system, particularly oligodendrocytes. In order to facilitate research on JC Virus, the LMMN has developed a system of human fetal neural multipotential cells, which can be differentiated into Oligodendrocytes. The differentiation process in humans is poorly understood, so we will perform RNA-seq at numerous time points during the differentiation process to understand gene expression in developing human oligodendrocytes. Our lab does not have the equipment to perform RNA-seq, so we need an outside group to provide this service.

Purpose and Objectives for the Procurement
The service provider will provide RNA Sequencing (RNA-Seq) for LMMN supplied RNA
templates. The RNA-Seq service includes: cDNA synthesis and library preparation, detection of
sequencing fragments using the Illumina platform, and an alignment of reads against a reference
genome. The primary objective of the study is to determine changes in gene expression and RNA splicing that occur during oligodendrocyte differentiation.

Capability Statement
Businesses that believe they possess the capabilities necessary to undertake this work should submit complete documentation of their capabilities to the Contract Specialist. The capabilities statement must specifically address each project requirement separately. Additionally, the capability statement should include 1) the total number of employees, 2) the professional qualifications of scientists, medical experts, and technical personnel as it relates to the above outlined requirements, 3) a description of general and specific facilities and equipment available, including computer equipment and software, 4) an outline of previous research projects that are similar to the project requirements in which the organization and proposed personnel have participated, and 5) any other information considered relevant to this program. The capability statement must not exceed 15 single sided or 7.5 double sided pages in length and using a 12-point font size minimum.

Interested organizations are required to identify their size standards in accordance with the Small Business Administration. The government requests that no proprietary or confidential business data be submitted in a response to this notice. However, responses that indicate the information therein is proprietary will be properly safeguarded for Government use only. Capability statements must include the name and telephone numbers of a point of contact having authority and knowledge to discuss responses with Government representatives. Capability statements in response to this market survey that do not provide sufficient information for evaluation will be considered non-responsive. When submitting this information, please reference the solicitation notice number.

All capability statements sent in response to this Sources Sought Notice must be submitted electronically (via email) to Andrea McGee, Contract Specialist – andrea.mcgee@nih.gov in MS Word within 15 calendar days of the date of this announcement. All responses must be received by the specified due date and time in order to be considered.

This notice does not obligate the Government to award a contract or otherwise pay for the information provided in the response. No proprietary, classified, confidential, or sensitive information should be included in your response. The Government reserves the right to use information provided by respondents for any purpose deemed necessary and legally appropriate. Any organization responding to this notice should ensure that its response is complete and sufficiently detailed to allow the Government to determine the organization’s qualifications to perform the work. Respondents are advised that the Government is under no obligation to acknowledge receipt of the information received or provide feedback to respondents with respect to any information submitted. After review of the responses received, pre-solicitation and solicitation notices may be published in Federal Business Opportunities. However, responses to this notice will not be considered adequate responses to a solicitation. The solicitation release date is pending. The Government intends to negotiate a fixed-price contract.
Due Date: June 11, 2013 – 3:00 P. M. Eastern Standard Time.

6001 Executive Boulevard
Room 4211 – MSC 9559
Bethesda, Maryland 20892
United States
9000 Rockville Pike
Bldg. 10
Bethesda, Maryland 20892
United States
Andrea McGee
Phone: 3014358781