Dr.Brian Johnson, Department of Entomology & Nematology, UC Davis
Monday, May 6, 2013
RNA-Seq is revolutionizing the study of gene expression. RNA-Seq has been shown to be quantitatively accurate over a larger range of expression levels than previous methods, such as microarrays, while also being more effective at identifying genes that show low expression levels. While the nuts and bolts of how best to use RNA-Seq are being addressed in a variety of contexts, major unresolved questions remain. With respect to insect studies, two questions of pressing concern are the sequencing depth necessary for documenting differential expression of genes of various classes, and the necessity, or lack thereof, of tissue specific RNA extractions. Here I will present results from two RNA-Seq studies exploring these issues. The primary results center on the utility of shallow RNA-Seq for many questions of interest to entomologists, and the necessity of tissue specificity for accurate quantification of gene expression. As extractions from body segments, and even whole insects, are common for insect RNA-Seq studies, the results are topical for those planning RNA-Seq studies.
Open to: Faculty/Staff Only
Sponsor: Entomology Department
Dr. Bradley White