The study of small RNAs provides us with a deeper understanding of the complexity of gene regulation within cells. Of the different types of small RNAs, the most important in mammals are miRNA, tRNA fragments and piRNAs. Using small RNA-seq analysis, we can study all small RNA types simultaneously, with the potential to detect novel small RNA types.
( A) Top panel with table showing the list of small RNAs detected (left) and genomic location (right). ( B) Middle panel shows abundance profile over the precursor (left), and secondary structure (right). This is an example of batch effect at the 3’ end (blue higher than brown) and disease effect at the 5’ end (solid lines higher than dashed lines). ( C) Bottom panel shows a table with the top most expressed sequences on the selected small RNA transcript. The index column is the sequence identifier that links the results to the original seqcluster output files.