For model species, the 3′ RNA-seq method might more accurately detect differential expression

December 5, 2016 Leave a comment 6,862 Views

One limitation of the widely used RNA-seq method is that long transcripts are represented by more reads than shorter transcripts, resulting in a biased estimation of expression levels. The 3′ RNA-seq method, which yields only one sequence per transcript, bypasses ...

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For model species, the 3′ RNA-seq method might more accurately detect differential expression

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