Marek’s disease is a contagious lymphoproliferative disease of chickens and typical model of viral oncogenesis. Mapping changes or different states over the course of infection for both host and pathogen would provide important insights into dynamic host-pathogen interactions. Researchers from ...Read More »
Alternative polyadenylation (APA) diversifies the 3′ termini of a majority of mRNAs in most eukaryotes, and is consequently inferred to have substantial consequences for the utilization of post-transcriptional regulatory mechanisms. Since conventional RNA-sequencing methods do not accurately define mRNA termini, ...Read More »
Why would a cell make abundant levels of 3’ UTR sequence and no coding sequence, since protein cannot be made without the coding sequence?
Widespread skewed expression of mRNA components correlate with fine tuning of protein production Rockefeller University – Science News – Long cast as a simple link between DNA and protein, messenger RNA has never offered much intrigue. But new research at ...Read More »
The 3′ untranslated region (3′ UTR) of mRNA contains elements that play regulatory roles in polyadenylation, localization, translation efficiency, and mRNA stability. Despite the significance of the 3′ UTR, there is no popular method for annotating 3′ UTRs and for ...Read More »
Improved Annotation of 3′ Untranslated Regions and Complex Loci by Combination of Strand-Specific Direct RNA Sequencing, RNA-Seq and ESTs
The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results of RNA-seq experiments where ...Read More »
This tutorial describes how to infer UTR length from RNA sequencing data. Use the GBrowse ruler function to measure the difference between a gene’s annotated start and the start of RNASeq reads. The difference estimates UTR length.Read More »