Gene expression profiling by high-throughput sequencing reveals qualitative and quantitative changes in RNA species at steady-state but obscures the intracellular dynamics of RNA transcription, processing and decay. Researchers at the Institute of Molecular Biotechnology, Vienna have developed thiol(SH)-linked alkylation for the metabolic ...
Read More »University of Cambridge researchers describe new method for revealing short-term changes in gene expression, alterations in RNA decay rates, and the kinetics of RNA processing
Cellular RNA levels are orchestrated by highly regulated processes involving RNA synthesis (transcription), processing (e.g., splicing, polyadenylation, transport), and degradation. Profiling these changes provides valuable information on the regulation of gene expression. Total cellular RNA is a poor template for ...
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