Cellular RNA levels are determined by transcription and decay rates, which are fundamental in understanding gene expression regulation. Measurement of these two parameters is usually performed...
Read More »Small RNA sequencing reveals metastasis-related microRNAs in lung adenocarcinoma
The majority of lung cancer deaths are caused by metastatic disease. MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression and miRNA dysregulation can contribute to metastatic progression. Here, researchers from Aarhus University used small RNA sequencing to profile the miRNA ...
Read More »Variation in gene expression is much larger than currently believed
RNA-Seq and gene expression microarrays provide comprehensive profiles of gene activity, but lack of reproducibility has hindered their application. A key challenge in the data analysis is the normalization of gene expression levels, which is currently performed following the implicit ...
Read More »Researchers discourage sample pooling in RNA-Seq experiments
Massively parallel cDNA sequencing (RNA-seq) experiments are gradually superseding microarrays in quantitative gene expression profiling. However, many biologists are uncertain about the validity of cost-saving sample pooling strategies for their RNA-seq experiments. Researchers from Aarhus University sequenced RNA-pools and compared their ...
Read More »Correction of gene expression data: performance-dependency on inter-replicate and inter-treatment biases
This report investigates for the first time the potential inter-treatment bias source of cell number for gene expression studies. Cell-number bias can affect gene expression analysis when comparing samples with unequal total cellular RNA content or with different RNA extraction ...
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