Tag Archives: Aarhus University

Researchers discourage sample pooling in RNA-Seq experiments

rna-seq

Massively parallel cDNA sequencing (RNA-seq) experiments are gradually superseding microarrays in quantitative gene expression profiling. However, many biologists are uncertain about the validity of cost-saving sample pooling strategies for their RNA-seq experiments. Researchers from Aarhus University sequenced RNA-pools and compared their ...

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Correction of gene expression data: performance-dependency on inter-replicate and inter-treatment biases

rn-seq

This report investigates for the first time the potential inter-treatment bias source of cell number for gene expression studies. Cell-number bias can affect gene expression analysis when comparing samples with unequal total cellular RNA content or with different RNA extraction ...

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