RNA-seq is a reference technology for determining alternative splicing at genome-wide level. Exon arrays remain widely used for the analysis of gene expression, but show poor validation rate with regard to splicing events. Commercial arrays that include probes within exon ...
Read More »Big Industry News – Thermo to buy Affy – Illumina launches liquid biopsy startup
Reuters – Thermo Fisher to buy Affymetrix for $1.3 billion Thermo Fisher Scientific Inc (TMO.N), the world’s largest maker of scientific instruments, said it agreed to buy Affymetrix Inc (AFFX.O) for $1.3 billion in cash, beefing up its genetic analysis ...
Read More »Microarrays give broader view of overall gene expression, while RNA-Seq demonstrates alternative splicing and specific pathways
Cell-free fetal RNA from the amniotic fluid supernatant of five euploid mid-trimester samples was divided and prepared in tandem for analysis by either the Affymetrix HG-U133 Plus 2.0 Gene Chip microarray or Illumina HiSeq. Transcriptomes were assembled and compared on ...
Read More »Comparison of RNA-Seq and Microarray in Transcriptome Profiling
To demonstrate the benefits of RNA-Seq over microarray in transcriptome profiling, researchers at Janssen Research & Development performed both RNA-Seq and microarray analyses on RNA samples from a human T cell activation experiment. In contrast to other reports, their analyses ...
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