Tag Archives: alternative polyadenylation

Alternative polyadenylation (APA) has been increasingly recognized as a crucial mechanism that contributes to transcriptome diversity and gene expression regulation. As RNA-seq has become a routine...

RNA sequencing (RNA-seq) is a high throughput technology that profiles gene expression in a genome-wide manner. RNA-seq has been mainly used for testing differential expression (DE) of transcripts between two conditions and has recently been used for testing differential alternative ...

DATE: June 28, 2017 – TIME: 9:00AM PDT, 12:00PM EDT, 6:00PM CEST SPONSORED BY: Lexogen Jernej Ule, PhD Group Leader, The Francis Crick Institute Gregor Rot, PhD Postdoctoral Researcher, Institute of Molecular Life Sciences University of Zurich ABSTRACT: Many RNA binding proteins ...

The recent emergence of alternative polyadenylation (APA) as an engine driving transcriptomic diversity has stimulated the development of sequencing methodologies designed to assess genome-wide polyadenylation events. The goal of these approaches is to enrich, partition, capture and ultimately sequence poly(A) ...

Cancer is a disease of the genome caused by oncogene activation and tumor suppressor gene inhibition. Deep sequencing studies including large consortia such as TCGA and ICGC identified numerous tumor-specific mutations not only in protein-coding sequences but also in non-coding ...

RNA-seq data analysis has revealed abundant alternative splicing in eukaryotic mRNAs. However, splicing is only one of many processing events that transcripts may undergo during their lifetime. Researchers from the Institute for Integrative Biology of the Cell, CNRS present here ...

A major objective of systems biology is to quantitatively integrate multiple parameters from genome-wide measurements. To integrate gene expression with dynamics in poly(A) tail length and adenylation site, researchers at Monash University developed a targeted next-generation sequencing approach, Poly(A)-Test RNA-sequencing. ...

Transcriptome-wide RNA structure probing coupled with NGS yields the RNA structurome. RNA structurome data tremendously improve prediction of in vivo RNA structure. RNA structurome data uncover relationships between RNA structure and gene regulation. RNA folds into intricate structures that enable ...

The human transcriptome comprises >80 000 protein-coding transcripts and the estimated number of proteins synthesized from these transcripts is in the range of 250 000 to 1 million. These transcripts and proteins are encoded by less than 20 000 genes, suggesting extensive regulation ...

Protein-coding genes with multiple alternative polyadenylation sites can generate mRNA 3’UTR sequences of different lengths, thereby causing the loss or gain of regulatory elements, which can affect stability, localization and translation efficiency. 3USS is a web-server developed with the aim ...