Tag Archives: amplification

As part of the process of preparing scRNA-seq libraries, a diverse template is typically amplified by PCR. During amplification, spurious chimeric molecules can be formed between molecules originating in different cells. While several computational and experimental strategies have been suggested ...

Currently quantitative RNA-Seq methods are pushed to work with increasingly small starting amounts of RNA that require PCR amplification to generate libraries. However, it is unclear how much noise or bias amplification introduces and how this effects precision and accuracy ...

AMSBIO announces RapidSeq – new convenient and cost-effective library sample preparation kits for Next Generation Sequencing (NGS) research and diagnostic work. The RapidSeq  Small RNA Sample Prep Kit is optimised for preparation of NGS libraries for subsequent cluster generation, using ...

Single-cell sampling with RNA-seq analysis plays an important role in reference laboratory; cytogenomic diagnosis for specimens on glass-slides or rare cells in circulating blood for tumor and genetic diseases; measurement of sensitivity and specificity in tumor-tissue genomic analysis with mixed-cells; ...