BigOmics Analytics has launched the web-based version of the Omics Playground, an extremely user-friendly platform that allows non-coders to analyse RNA-Seq, scRNA-seq, microarray and proteomics data types. Through it users can perform basic analysis, including sample clustering, pairwise...
Read More »Learn more about available bioinformatics tools and pipelines to analyze RNA sequencing data
Just as RNA itself has primary and secondary structure, RNA-Seq analysis has primary, Secondary and even tertiary steps. Which is the right tool at...
Read More »A computational workflow for the detection of DE genes and pathways from RNA-seq data using open source R software packages
In recent years, RNA sequencing (RNA-seq) has become a very widely used technology for profiling gene expression. One of the most common aims of RNA-seq profiling is to identify genes or molecular pathways that are differentially expressed (DE) between two ...
Read More »RNA-seq mixology – designing realistic control experiments to compare protocols and analysis methods
Carefully designed control experiments provide a gold standard for benchmarking new platforms, protocols and pipelines in genomics research. RNA profiling control studies frequently use the mixture design, which takes two distinct samples and combines them in known proportions to induce ...
Read More »QuickRNASeq lifts large-scale RNA-seq data analyses to the next level of automation and interactive visualization
RNA sequencing (RNA-seq), a next-generation sequencing technique for transcriptome profiling, is being increasingly used, in part driven by the decreasing cost of sequencing. Nevertheless, the analysis of the massive amounts of data generated by large-scale RNA-seq remains a challenge. Multiple ...
Read More »htsint – a Python library for sequencing pipelines that combines data through gene set generation
Sequencing technologies provide a wealth of details in terms of genes, expression, splice variants, polymorphisms, and other features. A standard for sequencing analysis pipelines is to put genomic or transcriptomic features into a context of known functional information, but the ...
Read More »RNA-Seq a hot topic at last month’s ISMB conference
Poster – A50 Gene expression analyses of RNA-Sequencing data across multiple cancers to identify Basal-like cancer subtypes Kevin Thompson, Mayo Clinic, United States Xiaojia Tang, Mayo Clinic, United States Jason Sinwell, Mayo Clinic, United States Peter Vedell, Mayo Clinic, United ...
Read More »Orione – a web-based framework for NGS analysis in microbiology
End-to-end NGS microbiology data analysis requires a diversity of tools covering bacterial resequencing, de novo assembly, scaffolding, bacterial RNA-Seq, gene annotation and metagenomics. However, the construction of computational pipelines that use different software packages is difficult due to a lack ...
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