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Tag Archives: Anton Dohrn

Annocript – a flexible pipeline for the annotation of transcriptomes also able to identify putative long noncoding RNAs

February 27, 2015 Leave a comment 3,415 Views

rna-seq

The eukaryotic transcriptome is composed of thousands of coding and long noncoding RNAs (lncRNAs). However, we lack a software platform to identify both RNA classes in a given transcriptome. Here researchers from the Anton Dohrn – Zoological Station introduce Annocript, ...

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    11 hours ago
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    DEBKS – a tool to detect differentially expressed circular RNA

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