Improvements in next-generation sequencing technologies have resulted in dramatically reduced sequencing costs. This has led to an explosion of "-seq"-based methods, of which RNA-seq for generating transcriptomic data is the most popular...
Read More »How well do RNA-Seq differential gene expression tools perform in higher eukaryotes?
RNA-seq experiments are usually carried out in three or fewer replicates. In order to work well with so few samples, Differential Gene Expression (DGE) tools typically assume the form of the underlying distribution of gene expression. A recent highly replicated ...
Read More »RNA-Seq enables a complete reannotation of the Arabidopsis thaliana reference genome
The flowering plant Arabidopsis thaliana is a dicot model organism for research in many aspects of plant biology. A comprehensive annotation of its genome paves the way for understanding the functions and activities of all types of transcripts, including mRNA, ...
Read More »A High Resolution Map of the Arabidopsis thaliana Developmental Transcriptome Based on RNA-seq Profiling
Arabidopsis thaliana is a long established model species for plant molecular biology, genetics and genomics, and studies of A.thaliana gene function provide the basis for formulating hypotheses and designing experiments involving other plants, including economically important species. A comprehensive understanding ...
Read More »An efficient method for simultaneous extraction of high-quality RNA and DNA from various plant tissues
Determination of gene expression is an important tool to study biological processes and relies on the quality of the extracted RNA. Changes in gene expression profiles may be directly related to mutations in regulatory DNA sequences or alterations in DNA ...
Read More »mirEX 2.0 – an integrated environment for expression profiling of plant microRNAs
MicroRNAs are the key post-transcriptional regulators of gene expression in development and stress responses. Thus, precisely quantifying the level of each particular microRNA is of utmost importance when studying the biology of any organism. The mirEX 2.0 web portal provides ...
Read More »Penn Scientists Identify Patterns of RNA Regulation in the Nuclei of Plants
When the human genome was first sequenced, experts predicted they would find about 100,000 genes. The actual number has turned out to be closer to 20,000, just a few thousand more than fruit flies have. The question logically arose: how ...
Read More »A multistep screening method to identify genes using evolutionary transcriptome of plants
A team led by researchers at the National Academy of Agricultural Science, Korea, have developed a multistep screening method to identify the genes in plants using microarrays and ribonucleic acid (RNA)-seq transcriptome data. This method describes the process for identifying ...
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