microRNAs (miRNA) are small, noncoding RNAs that bind to messenger RNAs (mRNAs) and regulate their activity. They are, therefore, important posttranscriptional regulators. In recent years it has become clear that miRNAs regulate large genetic networks, rather than single genes, and ...
Read More »Degradome Sequencing Enables Identification of ARGONAUTE/Small RNA Cleavage Sites
Researchers from the National Agricultural Research and Innovation Centre, Hungary describe a method that enables the high-throughput identification of cleaved mRNA targets of ARGONAUTE/small RNA complexes. The protocol is based on a modified 5′-rapid amplification of cDNA ends combined with deep sequencing of ...
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