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Tag Archives: Backtracked RNA polymerase

RNA-Seq sheds light on transcription fidelity

July 23, 2019 Leave a comment 1,750 Views

rna-seq

RNA transcription errors are transient, yet frequent, events that do have consequences for the cell. However, until recently we lacked the tools to empirically measure and study these errors...

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RNA-SEQ & COVID-19

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Recent RNA-SEQ Pubs

Screening and identification of autophagy-related biomarkers for oral squamous cell carcinoma (OSCC) via integrated bioinformatics analysis
10 April 2021
Drug-exposed cancer-associated fibroblasts facilitate gastric cancer cell progression following chemotherapy
10 April 2021
A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment
10 April 2021
LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B
10 April 2021
Bayesian estimation of cell type-specific gene expression with prior derived from single-cell data
10 April 2021
Automated quality control and cell identification of droplet-based single-cell data using dropkick
10 April 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells

    2 days ago
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    A versatile cell fixation-dissociation method for single-cell transcriptomics

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