Host-pathogen interactions are exceedingly complex because they involve multiple host tissues, often occur in the context of normal microflora, and can span diverse microenvironments. Although decades of gene expression studies have provided detailed insights into infection processes, technical challenges have ...
Read More »5′ Specific RNA-Seq – analyze specific classes of RNAs based upon the phosphorylation status of the 5′ end
Conventional high-throughput RNA sequencing (RNA-seq) uses massively parallel sequencing technology to analyze cDNA derived from total cellular RNA and is usually intended to survey the entire transcriptome. Often, however, it is preferable to derive sequence information from only a particular ...
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