Tag Archives: Berkeley

NMFP – a non-negative matrix factorization based preselection method to identify mRNA isoforms from RNA-seq data


The advent of next-generation RNA sequencing (RNA-seq) has greatly advanced transcriptomic studies, including system-wide identification and quantification of mRNA isoforms under various biological conditions. A number of computational methods have been developed to systematically identify mRNA isoforms in a high-throughput ...

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A method for extracting high-quality RNA from diverse plants for next-generation sequencing and gene expression analyses

To study expression in plants, high-quality RNA must be extracted in quantities sufficient for subsequent cDNA library construction. Field-based collections are often limited in quantity and quality of tissue and are typically preserved in RNAlater. Obtaining sufficient and high-quality yield ...

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