Don't Miss
  • Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source
  • 2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing
  • scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases
  • DEBKS – a tool to detect differentially expressed circular RNA
  • Single-cell RNA and protein expression in relation to cell cycle progression
  • Evaluating capture sequence performance for single-cell CRISPR activation experiments
  • Seven Bridges Selected by Pfizer to Support Data Management Solution for Single-Cell RNA Sequencing Data
  • Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes
  • Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq
  • Introduction to NGS – RNA-Seq Data Analysis – DNA Variant Calling

RNA-Seq BlogRNA-Seq Blog Transcriptome Research & Industry News

  • Home
  • News
    • Commentary
    • Headlines
    • Industry News
    • Grants / Funding
    • Information
    • Poll Results
    • Press Releases
    • Transcriptome Sequenced
  • Events
    • Conferences
    • Meetings
    • Webinars
    • Workshops
    • Projects
    • Local Events
  • Jobs
  • Technology
    • Methods
      • Workflow
      • RNA Extraction
      • Library Preparation
      • Sequencing Protocols
      • Statistical Analysis
      • Data Analysis
        • Analysis Pipelines
        • Annotation
        • Clouding Platforms
        • Data Normalization
        • Data Sets
        • Data Visualization
        • Databases
        • Expression and Quantification
        • Other Tools
        • Pathway Analysis
        • SNP Detection
        • Splicing and Junction Mapping
        • Statistical Analysis
        • Transcriptome Assembly Tools
        • Unspliced Mapping Tools
    • New Products
      • Our Advertisers
    • Patents
    • Presentations
    • Publications
    • Review Publications
    • Web Tools
  • Data Analysis
    • Analysis Pipelines
    • Annotation
    • Clouding Platforms
    • Data Normalization
    • Data Sets
    • Data Visualization
    • Databases
    • Expression and Quantification
    • Other Tools
    • Pathway Analysis
    • SNP Detection
    • Splicing and Junction Mapping
    • Statistical Analysis
    • Transcriptome Assembly Tools
  • Blog
  • Reader Posts
  • Contact
    • Administration
    • Contribute News
    • Subscribe
    • Advertise
    • Request for RNA-Seq Services

Tag Archives: bin definitions

Differential transcript usage from RNA-seq data – isoform pre-filtering improves performance of count-based methods

August 27, 2015 Leave a comment 5,218 Views

rna-seq

Large-scale sequencing of cDNA (RNA-seq) has been a boon to the quantitative analysis of transcriptomes. A notable application is the detection of changes in transcript usage between experimental conditions. For example, discovery of pathological alternative splicing may allow the development ...

Read More »

Stay Connected

RNA-SEQ & COVID-19

Publications Trend

Recent RNA-SEQ Pubs

Gill and liver transcriptomic responses of Achirus lineatus (Neopterygii: Achiridae) exposed to water-accommodated fraction (WAF) of light crude oil reveal an onset of hypoxia-like condition
1 March 2021
Deep sequencing of the Sanghuangporus vaninii transcriptome reveals dynamic landscapes of candidate genes involved in the biosynthesis of active compounds
1 March 2021
Integrating imaging and RNA-seq improves outcome prediction in cervical cancer
1 March 2021
Gut microbiota shape the inflammatory response in mice with an epithelial defect
1 March 2021
Selection and validation of reference genes for quantitative Real-time PCR analysis in Amana edulis
1 March 2021
Sphingosine-1-phosphate receptor 3 is implicated in BBB injury via the CCL2-CCR2 axis following acute intracerebral hemorrhage
1 March 2021

News in Pictures

rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq

recent visitors

Subscribe to the RNA-Seq Blog


Email Frequency

RNA-Seq Products & Services

Most Shared Posts

  • Bioinformatician, Post-doctoral candidate in Bioinformatics
    Google +1 logo   Twitter logo   Facebook logo
  • Potential drug target for dangerous E. coli infections identified by RNA sequencing
    Google +1 logo   Twitter logo   Facebook logo
  • Single cell RNA sequencing enables breakthrough study into the link between immunology and psychiatric disorders
    Google +1 logo   Twitter logo   Facebook logo
  • New map of the immune landscape in pancreatic cancer could guide future personalized immunotherapy treatments
    Google +1 logo   Twitter logo   Facebook logo
  • Single-cell genomics and spatial transcriptomics applied to liver disease biology
    Google +1 logo   Twitter logo   Facebook logo
  • Popular
  • Recent
  • Comments
  • rna-seq

    RPKM, FPKM and TPM, clearly explained

    July 22, 2015
  • rna-seq

    How to Analyze RNA-Seq Data?

    October 10, 2016
  • rna-seq

    Visualization of nucleotide substitutions in the (micro)transcriptome

    August 13, 2014
  • rna-seq

    Life Technologies Releases New Research Tool: Oncomine NGS RNA-Seq Gene Expression Browser

    April 8, 2014
  • rna-seq

    Comparison of TMM (edgeR), RLE (DESeq2), and MRN Normalization Methods

    October 6, 2016
  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    3 hours ago
  • rna-seq

    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

    4 hours ago
  • rna-seq

    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    1 day ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    1 day ago
  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    4 days ago
  • Ivan Molineris: If I understood well the annotation "ENSEMBL" used in this p...
  • Aashni: Nice article...
  • rna fixation: Wonderful article! We will be linking to this great article...
  • Protein Fixation: I was Searching Health blog commenting site the time I found...
  • SimPHARM.com: Great post, every thing is describe here very understandable...

Categories

Archives

What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

Recent News

  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    3 hours ago
  • rna-seq

    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

    4 hours ago
  • rna-seq

    scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases

    1 day ago
  • rna-seq

    DEBKS – a tool to detect differentially expressed circular RNA

    1 day ago
  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    4 days ago
  • rna-seq

    Evaluating capture sequence performance for single-cell CRISPR activation experiments

    4 days ago
  • rna-seq

    Seven Bridges Selected by Pfizer to Support Data Management Solution for Single-Cell RNA Sequencing Data

    4 days ago

Subscribe to the RNA-Seq Blog


Email Frequency

Social Networking Pages


RNA-Seq
  • 6,556 Followers
  • 2,079 Fans
© Copyright 2009-2020, All Rights Reserved. | Designed by Go Big - Online Presence Creations