Vicinal: a method for the determination of ncRNA ends using chimeric reads from RNA-seq experiments

March 21, 2014 Leave a comment 9,074 Views

Non-coding (nc)RNAs are important structural and regulatory molecules. Accurate determination of the primary sequence and secondary structure of ncRNAs is important for understanding their functions. During cDNA synthesis, RNA 3′ end stem-loops can self-prime reverse transcription, creating RNA-cDNA chimeras. Researchers ...

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