Tag Archives: biological variation

Understanding the differences between microarray and RNA-Seq technologies for measuring gene expression is necessary for informed design of experiments and choice of data analysis methods

rna-seq

Previous comparisons between microarrays and RNA-Seq have come to sometimes contradictory conclusions, which researchers from Princeton University suggest result from a lack of attention to the intensity-dependent nature of variation generated by the technologies. To examine this trend, they carried ...

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PROPER – Comprehensive Power Evaluation for Differential Expression using RNA-seq

rna-seq

RNA-seq has become a routine technique in differential expression (DE) identification. Scientists face a number of experimental design decisions including the sample size. The power for detecting differential expression is affected by several factors including the fraction of DE genes, ...

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Bitseq found to produce the most accurate measures of gene expression

rna-seq

RNA-sequencing data analysis method BitSeq developed by Academy Research Fellow Antti Honkela?s research group and University of Manchester researchers has been found to be the most accurate gene transcript expression estimation method in a large international assessment. The method is ...

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Technical variability is too high to ignore

RNA-seq is revolutionizing the way we study transcriptomes. mRNA can be surveyed without prior knowledge of gene transcripts. Alternative splicing of transcript isoforms and the identification of previously unknown exons are being reported. Initial reports of differences in exon usage, ...

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