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Tag Archives: Buffalo

Biocomputational identification and validation of novel microRNAs predicted from whole genome shotgun sequences

September 21, 2017 Leave a comment 3,619 Views

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MicroRNAs (miRNAs) are small (19-25 base long), non-coding RNAs that regulate post-transcriptional gene expression by cleaving targeted mRNAs in several eukaryotes. The miRNAs play vital roles in multiple biological and metabolic processes, including developmental timing, signal transduction, cell maintenance and ...

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ROS-Induced Hepatotoxicity under Cypermethrin: Involvement of the Crosstalk between Nrf2/Keap1 and NF-κB/iκB-α Pathways Regulated by Proteasome
12 April 2021
Dataset of the transcriptomes of <em>Urechis unicinctus</em> to identify differentially expressed genes (DEGs) under different temperature and exposure to open air
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Protective effects and molecular mechanisms of <em>Achyranthes bidentata</em> polypeptide k on Schwann cells
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Single Cell Label-Free Probing of Chromatin Dynamics During B Lymphocyte Maturation
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Transcriptomic Changes Induced by Deletion of Transcriptional Regulator <em>GCR2</em> on Pentose Sugar Metabolism in <em>Saccharomyces cerevisiae</em>
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Editorial: Genomic Characterization of Emerging Human Fungal Pathogens
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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