Tag Archives: California Institute of Technology

The allocation of a sequencing budget when designing single cell RNA-seq experiments requires consideration of the tradeoff between number of...

Single-cell sequencing experiments use short DNA barcode ‘tags’ to identify reads that originate from the same cell. In order to recover single-cell information from such experiments, reads must be...

RNA-seq has become the gold standard for mapping transcriptomes, profiling changes in splicing and measuring gene expression levels. The most widely used method for RNA-seq library construction is the dUTP approach. Although this approach provides high-quality strand-specific RNA-seq profiles, it ...

by Matt Fearer CAMBRIDGE, Mass. – The so-called central dogma of molecular biology—that DNA makes RNA which makes protein—has long provided a simplified explanation for how genetic information is deciphered and translated in living organisms. In reality, of course, the ...