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Tag Archives: cattle

RNA Sequencing (RNA-Seq) Reveals Extremely Low Levels of Reticulocyte-Derived Globin Gene Transcripts in Peripheral Blood From Horses (Equus caballus) and Cattle (Bos taurus)

August 28, 2018 Leave a comment 1,264 Views

RNA-seq has emerged as an important technology for measuring gene expression in peripheral blood samples collected from humans and other vertebrate species. In particular, transcriptomics analyses of whole blood can be used to study immunobiology and develop novel biomarkers of infectious disease. However, ...

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Transcriptomic analysis provides insights into candidate genes and molecular pathways involved in growth of Manila clam Ruditapes philippinarum
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De novo assembly of the transcriptome of scleractinian coral, Anomastraea irregularis and analyses of its response to thermal stress
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Yale researchers identify tumor reactive immune cells with RNA sequencing to help fight against advanced melanoma

    31 mins ago
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    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    23 hours ago
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    RNA sequencing brings hope for endangered wild cats

    24 hours ago
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    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

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    Uncovering the Anti-Myeloma Resistance Files

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