Tag Archives: cDNA libraries

Conventional high-throughput RNA sequencing (RNA-seq) uses massively parallel sequencing technology to analyze cDNA derived from total cellular RNA and is usually intended to survey the entire transcriptome. Often, however, it is preferable to derive sequence information from only a particular ...

The preparation and high-throughput sequencing of cDNA libraries from samples of small RNA is a powerful tool to quantify known small RNAs (such as microRNAs) and to discover novel RNA species. Interest in identifying the small RNA repertoire present in ...

Researchers at Stanford University present a simple molecular indexing method for quantitative targeted RNA sequencing, in which mRNAs of interest are selectively captured from complex cDNA libraries and sequenced to determine their absolute concentrations. cDNA fragments are individually labeled so ...