Multimodal analysis enables the simultaneous measurement of multiple modalities or the integration of data from multiple experiments. For a long time now, the advantages of sequencing mRNA (scRNA – Seq) in individual cells, to understand...
Read More »An enrichment system to facilitate isolation and molecular characterization of single cancer cells
Circulating tumor cells (CTCs) carry valuable biological information. While enumeration of CTCs in peripheral blood is an FDA-approved prognostic indicator of survival in metastatic prostate and other...
Read More »Host-Pathogen Transcriptomics by Dual RNA-Seq
Transcriptomics, i.e., the quantification of cellular RNA transcripts, is a powerful way to gauge the physiological state of either bacterial or eukaryotic cells under a given condition. However, traditional...
Read More »Improved RNA extraction from neurons
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, researchers from the RIKEN Institute present significant improvements to the protocol for isolation ...
Read More »RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures
Rare cell subtypes can profoundly impact the course of human health and disease, yet their presence within a sample is often missed with bulk molecular analysis. Single-cell analysis tools such as FACS, FISH-FC and single-cell barcode-based sequencing can investigate cellular ...
Read More »