Nuclear RNA Isolation and Sequencing

January 7, 2016 Leave a comment 5,877 Views

Most transcriptome studies involve sequencing and quantification of steady-state mRNA by isolating and sequencing poly (A) RNA. Although this type of sequencing data is informative to determine steady-state mRNA levels it does not provide information on transcriptional output and thus ...

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Nuclear RNA Isolation and Sequencing

RNA-Seq data suggests that exitron splicing represents an additional cancer-driving mechanism beyond genetic alterations

SPEAQeasy – a scalable pipeline for expression analysis and quantification for R/bioconductor-powered RNA-seq analyses

ac4C-seq – quantitative nucleotide resolution profiling of RNA cytidine acetylation

Trips-Viz: an environment for the analysis of public and user-generated ribosome profiling data

IsoSeek – unbiased and UMI-informed sequencing of cell-free miRNAs at single-nucleotide resolution

Cell-level metadata are indispensable for documenting single-cell RNA sequencing datasets

Strategies for detecting and identifying biological signals amidst the variation commonly found in RNA sequencing data