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Tag Archives: Census

Census – Single-cell mRNA quantification and differential analysis

January 25, 2017 Leave a comment 6,172 Views

rna-seq

Single-cell gene expression studies promise to reveal rare cell types and cryptic states, but the high variability of single-cell RNA-seq measurements frustrates efforts to assay transcriptional differences between cells. University of Washington researchers have developed the Census algorithm to convert ...

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    An RNA-Seq framework for profiling target selectivity and physiological effects of antisense peptide nucleic acids (PNAs)

    48 mins ago
  • rna-seq

    Single cell sequencing unravels molecular identity of rare renal cell types

    24 hours ago
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    1 day ago
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