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Tag Archives: CiBER-seq

CiBER-seq dissects genetic networks by quantitative CRISPRi profiling of expression phenotypes

December 11, 2020 Leave a comment 1,009 Views

rna-seq

CRISPR-Cas9 makes it easy to knock out or tweak a single gene to determine its effect on an organism or cell, or even another gene. But what if you could perform several thousand experiments at once, using CRISPR to tweak every gene in the genome...

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Recent RNA-SEQ Pubs

Development and Multi-Data Set Verification of an RNA Binding Protein Signature for Prognosis Prediction in Glioma
26 February 2021
Transcriptional and Proteomic Characterization of Telomere-Induced Senescence in a Human Alveolar Epithelial Cell Line
26 February 2021
The Role of Interleukin-33 in Head and Neck Squamous Cell Carcinoma Is Determined by Its Cellular Sources in the Tumor Microenvironment
26 February 2021
Bovine Pre-adipocyte Adipogenesis Is Regulated by bta-miR-150 Through mTOR Signaling
26 February 2021
Human Ubiquitin-Specific Peptidase 18 Is Regulated by microRNAs via the 3'Untranslated Region, A Sequence Duplicated in Long Intergenic Non-coding RNA Genes Residing in chr22q11.21
26 February 2021
TOXPANEL: A Gene-Set Analysis Tool to Assess Liver and Kidney Injuries
26 February 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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    Single-cell RNA and protein expression in relation to cell cycle progression

    19 hours ago
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