Don't Miss
  • New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens
  • RNA sequencing brings hope for endangered wild cats
  • Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs
  • Uncovering the Anti-Myeloma Resistance Files
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing
  • Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source
  • 2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing
  • scRNA-Seq reveals a new type of bone cell that may uncover therapeutic targets for a range of skeletal diseases
  • DEBKS – a tool to detect differentially expressed circular RNA
  • Single-cell RNA and protein expression in relation to cell cycle progression

RNA-Seq BlogRNA-Seq Blog Transcriptome Research & Industry News

  • Home
  • News
    • Commentary
    • Headlines
    • Industry News
    • Grants / Funding
    • Information
    • Poll Results
    • Press Releases
    • Transcriptome Sequenced
  • Events
    • Conferences
    • Meetings
    • Webinars
    • Workshops
    • Projects
    • Local Events
  • Jobs
  • Technology
    • Methods
      • Workflow
      • RNA Extraction
      • Library Preparation
      • Sequencing Protocols
      • Statistical Analysis
      • Data Analysis
        • Analysis Pipelines
        • Annotation
        • Clouding Platforms
        • Data Normalization
        • Data Sets
        • Data Visualization
        • Databases
        • Expression and Quantification
        • Other Tools
        • Pathway Analysis
        • SNP Detection
        • Splicing and Junction Mapping
        • Statistical Analysis
        • Transcriptome Assembly Tools
        • Unspliced Mapping Tools
    • New Products
      • Our Advertisers
    • Patents
    • Presentations
    • Publications
    • Review Publications
    • Web Tools
  • Data Analysis
    • Analysis Pipelines
    • Annotation
    • Clouding Platforms
    • Data Normalization
    • Data Sets
    • Data Visualization
    • Databases
    • Expression and Quantification
    • Other Tools
    • Pathway Analysis
    • SNP Detection
    • Splicing and Junction Mapping
    • Statistical Analysis
    • Transcriptome Assembly Tools
  • Blog
  • Reader Posts
  • Contact
    • Administration
    • Contribute News
    • Subscribe
    • Advertise
    • Request for RNA-Seq Services

Tag Archives: CircLigase

Small RNA-seq – The RNA 5′-end adapter ligation problem and how to circumvent it

May 16, 2019 Leave a comment 1,877 Views

rna-seq

The preparation of small RNA cDNA sequencing libraries depends on the unbiased ligation of adapters to the RNA ends. Small RNA with 5' recessed ends are poor substrates for enzymatic adapter ligation, but this 5' adapter ligation problem can...

Read More »

Stay Connected

RNA-SEQ & COVID-19

Publications Trend

Recent RNA-SEQ Pubs

Schistosoma mansoni alter transcription of immunomodulatory gene products following in vivo praziquantel exposure
3 March 2021
Establishment of a Cuscuta campestris-mediated enrichment system for genomic and transcriptomic analyses of 'Candidatus Liberibacter asiaticus'
3 March 2021
The transcriptome characteritics of vestibular organs from delayed endolymphatic hydrops patients(Menieres disease)
3 March 2021
Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis
3 March 2021
Transcriptomic and physiological responses of the tropical reef calcified macroalga Amphiroa fragilissima to elevated temperature
3 March 2021
The type 2 asthma mediator IL-13 inhibits SARS-CoV-2 infection of bronchial epithelium
3 March 2021

News in Pictures

rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq
rna-seq

recent visitors

Subscribe to the RNA-Seq Blog


Email Frequency

RNA-Seq Products & Services

Most Shared Posts

  • A new model to predict survival in colorectal cancer from DNA / RNA-Seq data
    Google +1 logo   Twitter logo   Facebook logo
  • Single-cell RNA sequencing points to potential single cause for psoriatic arthritis
    Google +1 logo   Twitter logo   Facebook logo
  • VIRTUS – a pipeline for comprehensive virus analysis from conventional RNA-seq data
    Google +1 logo   Twitter logo   Facebook logo
  • Researchers leverage whole transcriptome RNA sequencing and machine learning to improve lung cancer risk stratification
    Google +1 logo   Twitter logo   Facebook logo
  • Integrating single-cell RNA-seq and imaging with SCOPE-seq2
    Google +1 logo   Twitter logo   Facebook logo
  • Popular
  • Recent
  • Comments
  • rna-seq

    RPKM, FPKM and TPM, clearly explained

    July 22, 2015
  • rna-seq

    How to Analyze RNA-Seq Data?

    October 10, 2016
  • rna-seq

    Visualization of nucleotide substitutions in the (micro)transcriptome

    August 13, 2014
  • rna-seq

    Life Technologies Releases New Research Tool: Oncomine NGS RNA-Seq Gene Expression Browser

    April 8, 2014
  • rna-seq

    Comparison of TMM (edgeR), RLE (DESeq2), and MRN Normalization Methods

    October 6, 2016
  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    13 hours ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    14 hours ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    2 days ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

    2 days ago
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing

    2 days ago
  • RNA-Seq Blog: Thanks for letting us know. We have updated the link....
  • Yoni Sheinberger: The link doesn't work...
  • Ivan Molineris: If I understood well the annotation "ENSEMBL" used in this p...
  • Aashni: Nice article...
  • rna fixation: Wonderful article! We will be linking to this great article...

Categories

Archives

What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

Recent News

  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    13 hours ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    14 hours ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    2 days ago
  • rna-seq

    Uncovering the Anti-Myeloma Resistance Files

    2 days ago
  • Long term and short-term effects of RNA fixation on Single Cell RNA Sequencing

    2 days ago
  • rna-seq

    Single cell RNA sequencing opens new avenues for eradicating leukaemia at its source

    3 days ago
  • rna-seq

    2passtools: two-pass alignment using machine-learning-filtered splice junctions increases the accuracy of intron detection in long-read RNA sequencing

    3 days ago

Subscribe to the RNA-Seq Blog


Email Frequency

Social Networking Pages


RNA-Seq
  • 6,564 Followers
  • 2,079 Fans
© Copyright 2009-2020, All Rights Reserved. | Designed by Go Big - Online Presence Creations