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Tag Archives: cold stress

RNA-Seq uncovers key to cold tolerance of zebrafish

April 28, 2017 Leave a comment 2,929 Views

rna-seq

A team at National Taiwan University, led by Prof Shyh-Jye Lee of the Department of Life Science and director of the Research Center for Developmental Biology and Regenerative Medicine, have demonstrated that microRNAs (miRNAs) regulate the cold response of fish. ...

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Recent RNA-SEQ Pubs

Transcriptome analysis reveals the protective role of fructo-oligosaccharide in colonic mucosal barriers in exercise-induced stressed mice
22 April 2021
A Combination of RNA-Seq Analysis and Use of TCGA Database for Determining the Molecular Mechanism and Identifying Potential Drugs for GJB1 in Ovarian Cancer
22 April 2021
Transcriptomic analysis of immune cells in a multi-ethnic cohort of systemic lupus erythematosus patients identifies ethnicity- and disease-specific expression signatures
22 April 2021
Single-cell RNA sequencing in human lung cancer: Applications, challenges and pathway towards personalized therapy
22 April 2021
Targeting resistance to radiation-immunotherapy in cold HNSCCs by modulating the Treg-dendritic cell axis
22 April 2021
Defective Epstein-Barr Virus Genomes and Atypical Viral Gene Expression in B-Cell Lines Derived from Multiple Myeloma Patients
22 April 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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