Despite longstanding appreciation of gene expression heterogeneity in isogenic bacterial populations, affordable and scalable technologies for studying single bacterial cells have been limited. Although single-cell RNA sequencing (scRNA-seq) has...
Read More »Scalable and cost-effective ribonuclease-based rRNA depletion for transcriptomics
Bacterial RNA sequencing (RNA-seq) is a powerful approach for quantitatively delineating the global transcriptional profiles of microbes in order to gain deeper understanding of their physiology and function. Cost-effective bacterial RNA-seq requires...
Read More »RNA-Seq identifies microexons
The importance of RNA splicing in numerous cellular processes is well established. However, an underappreciated aspect is the ability of the spliceosome to recognize a set of very small (3-30 nucleotide, 1-10 amino acid) exons named microexons...
Read More »Featured RNA-Seq Job – Associate Research Scientist
Requisition Number: 0007907 Field(s) of Specialization: Position Title: Associate Research Scientist Department: 7574- JSB C2B2 Department Number: 757400X Location: Medical Center Summary Description Columbia University is looking for a highly motivated scientist to lead the analysis of large-scale genome and ...
Read More »Featured RNA-Seq Job – Staff Associate
Summary Description The Judith Sulzberger Genome Center at the Columbia University Medical Center is seeking a Staff Associate. This position will be in our next generation sequencing core, with emphasis on single cell RNA sequencing. The lab is fast growing, ...
Read More »phASER – Long range phasing and haplotypic expression from RNA sequencing
Haplotype phasing of genetic variants is important for clinical interpretation of the genome, population genetic analysis, and functional genomic analysis of allelic activity. Here researchers from Columbia University present phASER, a fast and accurate approach for phasing variants that are ...
Read More »mRIN – direct assessment of genome-wide and gene-specific mRNA integrity from large-scale RNA-sequencing data
The volume of RNA-Seq data sets in public repositories has been expanding exponentially, providing unprecedented opportunities to study gene expression regulation. Because degraded RNA samples, such as those collected from post-mortem tissues, can result in distinct expression profiles with potential ...
Read More »Featured RNA-Seq Job – Scientific Programmer
Job Description Columbia University is looking for a highly motivated data analyst to establish analytical protocols of large-scale cancer genome sequencing effort to improve personalized cancer care. The effort is a critical part of cancer precision medicine initiative at Columbia ...
Read More »Integrating transcriptome and genome sequencing
A practical webinar highlighting genetic data integration approaches featuring Dr. Tuuli Lappalainen. Explore the power of genome and transcriptome data integration, expand your understanding of genetic variants, and map future research applications. In this video you will learn how: Combining ...
Read More »Featured RNA-Seq Job – Chief Technician
Columbia University Job Description We seek an experienced Chief Technician to work closely with the Principal Investigator, Post Docs and Students in designing, carrying out, and coordinating research in the laboratory. Research in the A. Sepulveda laboratory is centered on ...
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