Identification and removal of sequencing artifacts produced by mispriming during reverse transcription in multiple RNA-seq technologies

June 29, 2018 Leave a comment 2,893 Views

The quality of RNA sequencing data relies on specific priming by the primer used for reverse transcription (RT-primer). Non-specific annealing of the RT-primer to the RNA template can generate reads with incorrect cDNA ends and can cause misinterpretation...

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Tag Archives: complementarity

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