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Tag Archives: corona virus

Single-cell RNA-seq reveals the potential risk of different human organs vulnerable to 2019-nCoV infection

March 24, 2020 Leave a comment 2,294 Views

rna-seq

It has been known that, the novel Coronavirus, 2019-nCoV, which is considered similar to SARS-CoV and originated from Wuhan (China), invades human cells via the receptor angiotensin converting enzyme...

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Recent RNA-SEQ Pubs

Full-length annotation with multistrategy RNA-seq uncovers transcriptional regulation of lncRNAs in cotton
25 February 2021
Epithelial Cells in 2D and 3D Cultures Exhibit Large Differences in Higher-order Genomic Interactions
25 February 2021
DEBKS: A Tool to Detect Differentially Expressed Circular RNA
25 February 2021
INPP5D expression is associated with risk for Alzheimer's disease and induced by plaque-associated microglia
25 February 2021
Identification of Key Genes in Sheep Fat Tail Evolution Based on RNA-seq
25 February 2021
Transcriptome Analysis Reveals Significant Difference in Gene Expression and Pathways Between Two Peanut Cultivars under Al Stress
25 February 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

Recent News

  • rna-seq

    Single-cell RNA and protein expression in relation to cell cycle progression

    4 hours ago
  • rna-seq

    Evaluating capture sequence performance for single-cell CRISPR activation experiments

    5 hours ago
  • rna-seq

    Seven Bridges Selected by Pfizer to Support Data Management Solution for Single-Cell RNA Sequencing Data

    6 hours ago
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    Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes

    1 day ago
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    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

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  • Introduction to NGS – RNA-Seq Data Analysis – DNA Variant Calling

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    Yale neurologists use RNA sequencing to identify consistent neuroinflammatory response in ICH patients

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