Experimental procedures for preparing RNA-seq and single-cell (sc) RNA-seq libraries are based on assumptions regarding their underlying enzymatic reactions. Here, researchers at the University of Warwick show that the fairness of these assumptions varies within libraries: coverage by sequencing reads ...
Read More »Experimental Design Considerations for Microbial RNA-seq
RNA-seq is being used increasingly for gene expression studies and it is revolutionizing the fields of genomics and transcriptomics. However, the field of RNA-seq analysis is still evolving. Now, researchers at the University of Tennessee, Knoxville specifically designed a study ...
Read More »The trade-off between increased multiplexing and decreasing sequencing depth in smallRNA-Seq
Small RNA sequencing can be used to gain an unprecedented amount of detail into the microRNA transcriptome. The relatively high cost and low throughput of sequencing bases technologies can potentially be offset by the use of multiplexing. However, multiplexing involves ...
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