Differential gene expression analysis is widely used to study changes in gene expression profiles between two or more groups of samples (e.g., physiological versus pathological conditions...
Read More »Monitoring SARS-CoV-2 using wastewater-based metatranscriptomic sequencing and droplet digital PCR to characterize RNA viromes
Alternative viral detection methods have been critical in the fight against COVID-19, helping to reduce the burden on our healthcare system. In a new study...
Read More »Direct RNA sequencing reveals SARS-CoV-2 m6A sites
The causative agent of COVID-19 pandemic, SARS-CoV-2, has a 29,903 bases positive-sense single-stranded RNA genome. RNAs exhibit about 150 modified bases that are essential for proper function.
Read More »scRNA-Seq can define host and viral transcriptional activity at the site of infection
Influenza A virus (IAV) and SARS-CoV-2 are pandemic viruses causing millions of deaths, yet their clinical manifestations are distinctly different...
Read More »Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping
Reverse phenotyping is a powerful tool to provide an integrated insight into cellular states of SARS-CoV-2...
Read More »DeepSARS – simultaneous diagnostic detection and genomic surveillance of SARS-CoV-2
The continued spread of SARS-CoV-2 and emergence of new variants with higher transmission rates and/or partial resistance to vaccines has...
Read More »Comprehensive investigation of COVID-19 RNA-sequencing dataset
The goal of this study is to reveal the hub genes and molecular mechanisms of the coronavirus disease 2019 (COVID-19) acute respiratory distress syndrome (ARDS) based on the genome-wide RNA...
Read More »Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through ‘reverse phenotyping’
The in vivo phenotypic profile of T cells reactive to severe acute respiratory syndrome (SARS)-CoV-2 antigens remains poorly understood. Conventional methods to detect antigen-reactive T cells require in...
Read More »UTHSC Hosting Open Data COVID-19 RNA Sequence Resource
Faculty members from the University of Tennessee Health Science Center have led the development of PubSeq, a global digital data repository of COVID-19 RNA sequences. PubSeq is open to all interested...
Read More »RNA-Seq shows that SARS-CoV-2 replication targets nasal ciliated cells early in COVID-19 infection
The establishment of nasal mucosal immunity against SARS-CoV-2 through a nasal vaccine could be the most efficient way to combat COVID-19...
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