CRISPR-Cas9 makes it easy to knock out or tweak a single gene to determine its effect on an organism or cell, or even another gene. But what if you could perform several thousand experiments at once, using CRISPR to tweak every gene in the genome...
Read More »LinTIMaT – a novel statistical learning approach for tracing cell lineage
Our body consists of numerous cells that are functioning continuously to keep us alive. Even though the cells in different organs contain almost similar DNA, they can perform different functions. While the cells perform different functions, their...
Read More »A Small RNA isolation and sequencing protocol and its application to assay CRISPR RNA biogenesis
Next generation high-throughput sequencing has enabled sensitive and unambiguous analysis of RNA populations in cells. Here, Stanford University researchers describe a method for isolation and strand...
Read More »Featured RNA-Seq Job – Staff Research Associate I/II
The Staff Research Associate I/II (SRA I/II) will be working in a cell and molecular biology laboratory. The SRA I/II will be an integral member of a research team that is using CRISPR libraries in mammalian cell screens applied to ...
Read More »CRISPR meets single-cell RNA sequencing in new screening method
CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences Genome editing using CRISPR/Cas9 “gene scissors” is a powerful tool for biological discovery and for identifying novel drug targets. In pooled CRISPR screens, a large number of cells ...
Read More »Perturb-seq – Enables Large-Scale Analysis of Complex Genetic Interactions Using CRISPR-Based Gene Perturbation and Single-Cell RNA Sequencing
Researchers from UC San Francisco and the Broad Institute of MIT and Harvard have developed a new method for performing high-throughput functional screening of complex genetic interactions and resulting phenotypes in single cells, which they have dubbed “Perturb-seq.” The findings ...
Read More »RNA-Seq Post-doc Postion Available
Postdoctoral Fellow – Functional Genomics of Immuno-Oncology Description: 2015 will be the year of the AbbVie Postdoctoral Program launch. We are looking for the incoming class to join AbbVie as the company is positioning itself for another significant year of ...
Read More »Investigating CRISPR RNA Biogenesis and Function Using RNA-Seq
The development of deep sequencing technology has greatly facilitated transcriptome analyses of both prokaryotes and eukaryotes. RNA-sequencing (RNA-seq), which is based on massively parallel sequencing of cDNAs, has been used to annotate transcript boundaries and revealed widespread antisense transcription as ...
Read More »Gene-Editing Guide
from Harvard Univeristy by By SUE McGREEVEY New method identifies genome-wide off-target effects of CRISPR-Cas Harvard Medical School investigators at Massachusetts General Hospital have developed a method for detecting unwanted DNA breaks—across the entire genome of human cells—induced by the ...
Read More »DrugTargetSeqR: a genomics- and CRISPR-Cas9-based method to analyze drug targets
To identify physiological targets of drugs and bioactive small molecules, researchers at The Rockefeller University developed an approach, named DrugTargetSeqR, which combines high-throughput sequencing, computational mutation discovery and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9-based genome editing. They applied this ...
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