omiRas – A MicroRNA-Seq Data Analysis Pipeline

April 30, 2015 Leave a comment 10,620 Views

To identify circulating miRNA biomarkers for AD, researchers from Meiji Pharmaceutical University, Japan reanalyzed a publicly available small RNA-Seq dataset, composed of blood samples derived from 48 AD patients and 22 normal control (NC) subjects, by a simple web-based miRNA ...

Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

September 19, 2014 Comments Off on Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing 5,963 Views

There are several protocols and kits for the extraction of circulating RNAs from plasma with a following quantification of specific genes via RT-qPCR. Due to the marginal amount of cell-free RNA in plasma samples, the total RNA yield is insufficient ...

RNA-Seq with iReport and InSilico DB

September 16, 2014 Comments Off on RNA-Seq with iReport and InSilico DB 5,082 Views

RNA-Seq, or transcriptome sequencing, continues to be an exciting way to explore gene expression using next-generation sequencing (NGS). But for many researchers, the interpretation of RNA-Seq data remains a daunting task because they are using spreadsheets to read transcript names ...

Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

August 28, 2014 Comments Off on Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation 12,168 Views

The collborators at the SEQC/MAQC-III Consortium present primary results from the Sequencing Quality Control (SEQC) project, coordinated by the US Food and Drug Administration. Examining Illumina HiSeq, Life Technologies SOLiD and Roche 454 platforms at multiple laboratory sites using reference ...

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Tag Archives: data analysis pipeline

omiRas – A MicroRNA-Seq Data Analysis Pipeline

Optimization of Extraction of Circulating RNAs from Plasma – Enabling Small RNA Sequencing

RNA-Seq with iReport and InSilico DB

Study finds RNA-Seq, like microarrays, only accurate for relative expression, not absolute quantitation

AAV-Perturb-seq – adeno-associated virus (AAV)-mediated direct in vivo single-cell CRISPR screening

capTEs – Cas9-assisted profiling transposable elements expression sequencing

eSPET-seq – genome-wide probing of eukaryotic nascent RNA structure

DISCERN – deep single-cell expression reconstruction for improved cell clustering and cell subtype and state detection

Advancements in genome sequencing propel global RNA sequencing market growth to 2030

VAX-seq – mRNA vaccine quality analysis using RNA sequencing

Single-cell RNA-sequencing integration analysis revealed immune cell heterogeneity in five human autoimmune diseases