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Tag Archives: DESCEND

Gene expression distribution deconvolution in single-cell RNA sequencing

June 28, 2018 Leave a comment 4,603 Views

rna-seq

Single-cell RNA sequencing (scRNA-seq) enables the quantification of each gene’s expression distribution across cells, thus allowing the assessment of the...

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Recent RNA-SEQ Pubs

Identification of RNAs bound by Hfq reveals widespread RNA partners and a sporulation regulator in the human pathogen Clostridioides difficile
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GPR126 regulates colorectal cancer cell proliferation by mediating HDAC2 and GLI2 expression
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Sex-specific expression and DNA methylation in a species with extreme sexual dimorphism and paternal genome elimination
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Single-Cell RNA Sequencing of the Adult Mammalian Heart-State-of-the-Art and Future Perspectives
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Comparative proteomic and transcriptomic analysis reveals high pH-induced expression signatures of Chinese shrimp Fenneropenaeus chinensis
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    Single-cell mapper (scMappR) – using scRNA-seq to infer the cell-type specificities of differentially expressed genes

    19 hours ago
  • rna-seq

    Featured RNA-Seq Job – Staff Bioinformatics Scientist, RNA-Seq

    20 hours ago
  • Introduction to NGS – RNA-Seq Data Analysis – DNA Variant Calling

    2 days ago
  • rna-seq

    Yale neurologists use RNA sequencing to identify consistent neuroinflammatory response in ICH patients

    2 days ago
  • rna-seq

    Resolve Biosciences’ Molecular Cartography platform detects specific gene expression patterns in SARS-CoV-2 not seen with existing single-cell RNA-seq technologies

    2 days ago
  • rna-seq

    Molecular Stethoscope publishes human proof-of-concept study for its cell-free mRNA based NGS liquid biopsy technology platform

    2 days ago
  • rna-seq

    Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

    3 days ago

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