Tag Archives: deseq

Assessment of single cell RNA-seq normalization methods

rna-seq

UCSD researchers have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Their analyses showed that methods considering spike-in ERCC RNA molecules significantly outperformed those not considering ERCCs. This work ...

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RNA-Seq analysis in SeqMonk with DESeq

rna-seq

This video shows a walk-through of a full 2-condition, 3-replicate RNA-Seq experiment, from loading the data, through QC, quantitation and differential expression analysis using both DESeq2 and SeqMonk’s own Intensity Difference filter. http://www.bioinformatics.babraham.ac.uk/projects/seqmonk/

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