Tag Archives: differential gene expression

There is a critical need for standard approaches to assess, report and compare the technical performance of genome-scale differential gene expression experiments. Here, an international team led by researchers at the NIST assess technical performance with a proposed standard ‘dashboard’ ...

RNA-seq is a methodology for RNA profiling based on next-generation sequencing that enables to measure and compare gene expression patterns at unprecedented resolution. Although the appealing features of this technique have promoted its application to a wide panel of transcriptomics ...

from Genetic Engineering News RNA sequencing (RNA-seq), a means of depicting the transcriptome, is being used with increasing frequency to characterize a growing array of conditions—everything from prenatal birth defects to disorders of the elderly. Yet the technique, which is ...

RNA-Seq, or transcriptome sequencing, continues to be an exciting way to explore gene expression using next-generation sequencing (NGS). But for many researchers, the interpretation of RNA-Seq data remains a daunting task because they are using spreadsheets to read transcript names ...

The concordance of RNA-sequencing (RNA-seq) with microarrays for genome-wide analysis of differential gene expression has not been rigorously assessed using a range of chemical treatment conditions. Here a team led by researchers atuse at the US FDA comprehensive study design ...

  Date: Tuesday, September 23, 2014, 10:00 am – 12:00 pm Location: 402 Walter Instructor(s):   This lecture will provide a basic overview of DGE analysis from RNA-Seq data. Lecture Topics: – RNA-Seq vs Microarray – Experimental Design – Sequencing ...

Next generation sequencing has made it possible to perform differential gene expression studies in non-model organisms. For these studies, the need for a reference genome is circumvented by performing de novo assembly on the RNA-seq data. However, transcriptome assembly produces ...

This video demonstrates how to use the NIAID HPC Web to do a differential gene expression analysis using Cuffdiff with known annotated genes. Output results are discussed.

Fast algorithms and well-arranged visualizations are required for the comprehensive analysis of the ever-growing size of genomic and transcriptomic next generation sequencing (NGS) data. ReadXplorer is a software offering straightforward visualization and extensive analysis functions for genomic and transcriptomic DNA ...

from CoreGenomics by James Hadfield I’m going to start this post by saying something that may be controversial “splicing analysis is a niche, strandedness is useful in a minority of cases and that most RNA-seq users are only interested in ...