Next-generation sequencing provides useful information about gene mutations, gene expression, epigenetic modification, microRNA expression, and copy number variations. More and more computing tools have been developed to analyze this large quantity of information. However, to test and find suitable analytical ...
Read More »DeTCT – a purely quantitative digital gene expression sample processing and analysis package
Many RNA-seq methods are excellent tools for in-depth mRNA expression analysis of small numbers of samples and provide information on the entire RNA molecule, alternative splicing and the quantity of transcript. However, they require an involved library preparation and often ...
Read More »BrAD-seq (Breath Adapter Directional sequencing) – a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction
Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a ...
Read More »Digital Gene Expression (DGE) is a valuable tool for gene discovery, quantification and annotation
The freshwater planarian Schmidtea mediterranea is recognised as a valuable model for research into adult stem cells and regeneration. With the advent of the high-throughput sequencing technologies, it has become feasible to undertake detailed transcriptional analysis of its unique stem ...
Read More »trieFinder – an efficient program for annotating digital gene expression (DGE) tags
Quantification of a transcriptional profile is a useful way to evaluate the activity of a cell at a given point in time. Although RNA-Seq has revolutionized transcriptional profiling, the costs of RNA-Seq are still significantly higher than microarrays, and often ...
Read More »Qlucore Omics Explorer Makes RNA-Seq Analysis Simple
RNA-seq data can now be analyzed just as easily as microarray data In recent years, transcriptomic profiling via next generation sequencing (RNA-seq) has emerged as both a technical and cost-effective alternative to arrays. Qlucore Omics Explorer 3.0 supports direct import ...
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