Tag Archives: Direct RNA sequencing

Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3’ untranslated regions (UTRs). The expression of alternative 3’ UTR isoforms is controlled in a gene-specific manner by environmental cues and is dysregulated in cancer. ...

Alternative polyadenylation is commonly examined using cDNA sequencing, which is known to be affected by template-switching artifacts. However, the effects of such template-switching artifacts on alternative polyadenylation are generally disregarded, while alternative polyadenylation artifacts are attributed to internal priming. Researchers ...

Current technologies for targeted characterization and manipulation of viral RNA primarily involve amplification or ultracentrifugation with isopycnic gradients of viral particles to decrease host RNA background. The former strategy is non-compatible for characterizing properties innate to RNA strands such as ...

Direct sequencing of RNA molecules such as virus genomes could help to unpick role of mysterious chemical modifications in genetic material. The genome of the flu virus has been fully sequenced in its native RNA form for the first time. ...

SeqLL, LLC announced today that it has been awarded a Phase I SBIR grant in the amount of $223,465 by the NIH/National Human Genome Research Institute to advance the development of Direct RNA Sequencing (DRS™) technology on SeqLL’s True Single ...

“Parts of the genome remain hidden, or are extremely hard to analyze with current methods,” said Jeffery Schloss, Ph.D., director of the Division of Genome Sciences at the National Human Genome Research Institute (NHGRI). “If we can’t see what’s there ...

Ribonucleic acid sequencing can allow us to monitor the RNAs present in a sample. This enables us to detect the presence and nucleotide sequence of viruses, or to build a picture of how active transcriptional processes are changing — information ...

Purpose This Funding Opportunity Announcement (FOA) solicits R01 grant applications to develop novel technologies that will enable at least one order of magnitude improvement in DNA sequencing, and practical methods for direct RNA sequencing.  Advances in genomics and more broadly ...

Narayanan Raghupathy, Ph.D., The Jackson Laboratory April 22, 2014 | 4 p.m. Kinne Library, Morris Building Direct RNA sequencing of genes with polymorphisms can provide quantitative estimates of maternal and paternal alleles’ expression, not just the total gene expression. Narayanan ...