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Tag Archives: DNA library preparation

REcount – measuring sequencer size bias using

May 2, 2019 Leave a comment 1,704 Views

rna-seq

Quantification of DNA sequence tags from engineered constructs such as plasmids, transposons, or other transgenes underlies many functional genomics measurements. Typically, such measurements rely on PCR followed by next...

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Recent RNA-SEQ Pubs

Neurodevelopmental and transcriptomic effects of CRISPR/Cas9-induced somatic <em>orco</em> mutation in honey bees
5 March 2021
RNA helicase DDX5 acts as a critical regulator for survival of neonatal mouse gonocytes
5 March 2021
Transcriptomic data showing differentially expressed genes between Notch3 and Notch4 deleted mice
5 March 2021
Comparative transcriptome analysis of roots, stems, and leaves of <em>Pueraria lobata</em> (Willd.) Ohwi: identification of genes involved in isoflavonoid biosynthesis
5 March 2021
The long non-coding RNA MEG3 plays critical roles in the pathogenesis of cholesterol gallstone
5 March 2021
Evaluation of key miRNAs during early pregnancy in Kazakh horse using RNA sequencing
5 March 2021

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    RPKM, FPKM and TPM, clearly explained

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    2 days ago
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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


RNA-Seq

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    Learn more about available bioinformatics tools and pipelines to analyze RNA sequencing data

    18 hours ago
  • rna-seq

    New search engine for single cell atlases

    19 hours ago
  • rna-seq

    Yale researchers identify tumor reactive immune cells with RNA sequencing to help fight against advanced melanoma

    20 hours ago
  • rna-seq

    New algorithm identifies ‘escaping’ cells in single-cell CRISPR screens

    2 days ago
  • rna-seq

    RNA sequencing brings hope for endangered wild cats

    2 days ago
  • rna-seq

    Mim-tRNAseq – A method that accurately measures the abundance and modification status of different tRNAs

    3 days ago
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    Uncovering the Anti-Myeloma Resistance Files

    3 days ago

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