A challenge for single-cell genomic studies in kidney and other solid tissues is generating a high-quality single-cell suspension that contains rare or difficult-to-dissociate cell types and is free of both RNA degradation and artifactual transcriptional stress...
Read More »Taming the transcriptome with RNA-Seq
There’s a new RNA-seq in town. Although the old RNA-seq administered a certain rough justice, profiling RNA species well enough to support a relatively crude conception of the transcriptome, the new RNA-seq is more refined...
Read More »DroNc-Seq – Single-nucleus RNA sequencing, droplet by droplet
DroNc-Seq — a technology that merges single-nucleus RNA sequencing with microfluidics — brings new scale to gene expression studies in complex tissues. from The Broad Institute by Tom Ulrich – Last year Broad researchers described a single-nucleus RNA sequencing method called sNuc-Seq. This ...
Read More »Dolomite Bio introduces new single nucleus RNA-Seq chip for DroNc-Seq
Dolomite Bio is pleased to announce the launch of a new chip for DroNc-Seq1, enabling high throughput single nucleus RNA-Seq using droplet microfluidics. The single nucleus RNA-Seq chip – based on the company’s established scRNA-Seq chip – is designed to ...
Read More »DroNc-Seq – Massively parallel single nucleus RNA – Seq
Single nucleus RNA-Seq (sNuc-Seq) profiles RNA from tissues that are preserved or cannot be dissociated, but does not provide the throughput required to analyse many cells from complex tissues. Here, Researchers from the Broad Institute of MIT and Harvard develop ...
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