Single-cell RNA sequencing offers snapshots of whole transcriptomes but obscures the temporal RNA dynamics. Researchers from the University of Pennsylvania have developed single-cell metabolically labeled new RNA tagging sequencing (scNT-seq), a method for massively parallel analysis of newly transcribed and ...
Read More »Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing
Single-cell RNA-seq reveals the cellular heterogeneity inherent in the population of cells, which is very important in many clinical and research applications. Recent advances in droplet microfluidics have achieved the automatic isolation, lysis...
Read More »Single-cell barcoding and sequencing using droplet microfluidics
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the unbiased diversity of cells. Droplet microfluidics is among the most promising candidates for ...
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