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Tag Archives: DSRG

Evaluation of commercially available RNA amplification kits at sub nanogram input amounts of total RNA for RNA-Seq

May 27, 2014 Leave a comment 6,818 Views

rna-seq

Multiple recent publications on RNA-Seq have demonstrated the power of next generation sequencing technologies in whole transcriptome analysis. The vendor specific protocols used for RNA library construction typically require at least 100ng of total RNA. However, under certain conditions such ...

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Recent RNA-SEQ Pubs

ROS-Induced Hepatotoxicity under Cypermethrin: Involvement of the Crosstalk between Nrf2/Keap1 and NF-κB/iκB-α Pathways Regulated by Proteasome
12 April 2021
Dataset of the transcriptomes of <em>Urechis unicinctus</em> to identify differentially expressed genes (DEGs) under different temperature and exposure to open air
12 April 2021
Protective effects and molecular mechanisms of <em>Achyranthes bidentata</em> polypeptide k on Schwann cells
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Single Cell Label-Free Probing of Chromatin Dynamics During B Lymphocyte Maturation
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Transcriptomic Changes Induced by Deletion of Transcriptional Regulator <em>GCR2</em> on Pentose Sugar Metabolism in <em>Saccharomyces cerevisiae</em>
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Editorial: Genomic Characterization of Emerging Human Fungal Pathogens
12 April 2021

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What is RNA-Seq?

long RNAs are first converted into a library of cDNA fragments through either RNA fragmentation or DNA fragmentation. Sequencing adaptors (blue) are subsequently added to each cDNA fragment and a short sequence is obtained from each cDNA using high-throughput sequencing technology. The resulting sequence reads are aligned with the reference genome or transcriptome, and classified as three types: exonic reads, junction reads and poly(A) end-reads. These three types are used to generate a base-resolution expression profile for each gene. Nat Rev Genet 10(1):57-63 (2009)


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  • rna-seq

    First use of both bulk and single-cell RNA sequencing of human bladder tumors to study resistance to immunotherapy

    22 mins ago
  • rna-seq

    Post-doc position available – transcriptome bioinformatics and RNA sequencing

    23 hours ago
  • rna-seq

    Introduction to scRNA-Seq Analysis

    1 day ago
  • rna-seq

    NanoSINC-seq dissects the isoform diversity in subcellular compartments of single cells

    4 days ago
  • rna-seq

    A versatile cell fixation-dissociation method for single-cell transcriptomics

    4 days ago
  • rna-seq

    RNAStructViz – graphical base pairing analysis

    5 days ago
  • rna-seq

    Spatial mapping of COVID-19 and H1N1 influenza show differential transcriptional signatures

    5 days ago

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