A Postdoctoral position in computational single-cell genomics is available in the Statistical Genomics and Systems Genetics group at our newly established location as part of the Genome Biology Unit at EMBL Heidelberg in Germany...
Read More »Single Cell RNA Sequencing – Workshop
Location & dates EMBL Heidelberg, Germany 15 - 18 May 2018 Deadlines Application - 20 Feb 2018 Course Overview The goal of this course is to teach the fundamentals of NGS library preparation...
Read More »Mirnovo – genome-free prediction of microRNAs from small RNA sequencing data and single-cells using decision forests
The discovery of microRNAs (miRNAs) remains an important problem, particularly given the growth of high-throughput sequencing, cell sorting and single cell biology. While a large number of miRNAs have already been annotated, there may well be large numbers of miRNAs ...
Read More »Power analysis of single-cell RNA-sequencing experiments
Single-cell RNA sequencing (scRNA-seq) has become an established and powerful method to investigate transcriptomic cell-to-cell variation, thereby revealing new cell types and providing insights into developmental processes and transcriptional stochasticity. A key question is how the variety of available protocols ...
Read More »Je – a suite of tools that accommodates complex barcoding strategies including unique molecular identifiers (UMIs)
The yield obtained from next generation sequencers has increased almost exponentially in recent years, making sample multiplexing common practice. While barcodes (known sequences of fixed length) primarily encode the sample identity of sequenced DNA fragments, barcodes made of random sequences ...
Read More »Introductory Course – Next Generation Sequencing: RNA Sequencing Library Preparation
EMBL Introductory Course Next Generation Sequencing: RNA Sequencing Library Preparation EMBL Heidelberg, Germany Wednesday 28 January – Friday 30 January 2015 Application closed Latest News Fellowships have been allocated. Successful applicants have been notified via email. The final programme is ...
Read More »Reduction of Gene Expression Variability from Single Cells to Populations follows Simple Statistical Laws
Recent studies on single cells and population transcriptomics have revealed striking differences in global gene expression distributions. Single cells display highly variable expressions between cells, while cell populations present deterministic global patterns. The mechanisms governing the reduction of transcriptome-wide variability ...
Read More »DESeq2 – moderated estimation of fold change and dispersion for RNA-Seq data
In comparative high-throughput sequencing assays, a fundamental task is the analysis of count data, such as read counts per gene in RNA-seq, for evidence of systematic changes across experimental conditions. Small replicate numbers, discreteness, large dynamic range and the presence ...
Read More »Upcoming Workshop – RNA Sequencing Library Preparation
EMBL Introductory Course EMBL Heidelberg, Germany Wednesday 28 January – Friday 30 January 2015 Application deadline: Sunday 14 December 2014 Abstract deadline: Sunday 14 December 2014 Application deadline: 14 December 2014. We only accept online registrations. The course is limited ...
Read More »Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples
Researchers at the Broad Institute have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. This method uses targeted RNase H-based digestion ...
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