Tag Archives: ERCC

Deep sequencing is a way to battle sequencing artifacts, and it might seem to obviate time-consuming controls. But, given today’s large-scale studies, standards and controls “provide confidence that your measurement system is working the way you want it to, the ...

To assess the performance of the Oxford Nanopore Technologies MinION sequencing platform, researchers from McGill University sequenced cDNAs from the External RNA Controls Consortium (ERCC) RNA Spike-In mix. This mix mimics mammalian mRNA species and consists of 92 polyadenylated transcripts ...

UCSD researchers have assessed the performance of seven normalization methods for single cell RNA-seq using data generated from dilution of RNA samples. Their analyses showed that methods considering spike-in ERCC RNA molecules significantly outperformed those not considering ERCCs. This work ...

Highly multiplexed assays for quantitation of RNA transcripts are being used in many areas of biology and medicine. Using data generated by these transcriptomic assays requires measurement assurance with appropriate controls. Methods to prototype and evaluate multiple RNA controls were ...

RNA is complex, and its sequencing is even more complex. One of the biggest complications originates from the fact that RNA consists of numerous variations such as alternative splicing and isoforms which are introduced during the process of transcription and ...

A new, innovative “dashboard” from the National Institute of Standards and Technology (NIST) won’t help you drive your car, but it will help enable reproducible research in biology. Artist’s conception of an RNA polymerase (globular molecule) creating strands of RNA ...