Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, researchers from the RIKEN Institute present significant improvements to the protocol for isolation ...
Read More »Experimental approaches commonly used for single-cell gene expression profiling
Single-cell gene expression profiling was pioneered more than 20 years ago using exponential amplification of cDNAs by PCR and linear amplification by in vitro transcription. These protocols, as well as the subsequent availability of high-density microarrays, led to the application ...
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